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To examine the structural properties of the proteoglycan (GMPG, Ganoderma lucidum mycelial proteoglycan) obtained from mycelia in Ganodermu lucidum IY009, we obtained the low and high molecular proteoglycan by ultrafiltration and sepharose CL-4B column chromatography. The physicochemical properties of these fractions were as follows. When the proteoglycan separated by ultrafiltration and sepharose CL-4B column chromatography, its was not fractionated completely The molecular weight of high molecular proteoglycan by the gel column chromatography (CH) was 250 kD and 2,000 kD, and low molecular proteoglycan was 12 kD. The total carbohydrate was consisted of 75.7% (UH) and 96.7% (CH), and the low fraction was 72.7% (UL) and 87.1% (CL), respectively. The sugar of high and low molecular proteoglycan composed of glucose, mannose, fructose, galactose, xylose, ribose and arabinose. Glucose contents of all fraction were ranged from 46.9%∼82.4% of the total sugar and the ratio of a and β-glucose was 0.84∼1.14, and its indicated the proteoglycan to be β-glucan. Amino acids pattern showed that the fractions contained a large amount of aspartic acid, glutamic acid, alanine and leucine. These fractions showed the characteristics of IR absorption for β-glucan at 890㎝^(-1) and ^(13)C-NMR spectroscopy showed the presence of the β-1,3-gluten and a β-1,6-glucan.
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